Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. Inst. Isolated from sub-artic decomposing wood. Micrococcus roseus 8. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. The analysis of the DNA with BaseSpace revealed unreliable data. Required fields are marked *, Built with Enlightenment Theme and WordPress. Table 2: Antibiotic Susceptibility Results. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. This genus is relatively young and is currently growing with many of the species being described within the last ten years. The cell walls of Gram positive bacteria differ structurally from the cell … The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Abstract. Planococcus, Staphylococcus,,
the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. Planococcus. have atypical Gram positive cell walls. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Euzeby, J. P., & Parte, A. C. (2017, April 1). The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic Planococcus citreus is Gram stain positive and aerobic. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. (2008). The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Micrococcus kristinae 4. It is urease and catalase positive. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. Arb. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. Further analysis of the genomic information was necessary. These results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme respectively. The microbe was susceptible to all eight antibiotics tested. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The sample was collected from a dry piece of wood. The gram-stain was repeated three times before a successful stain was accomplished. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Stomatococcus
To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. The quadratic streak was used 4 times to ensure a pure culture. Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. nov. M. Engelhardt , K. Daly , R. Swannell , I. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). BaseSpace has many apps that provide different information about the genomic data. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. 1a). The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. Halotolerant will grow in nutrient agar
Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. It was identified in 1894, and was only approved in 19806. Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. Gram stain. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. penetrates wall of decolorized gram - cells gram negative appear red. The gram-stain was repeated three times before a successful stain was accomplished. The cells were measured to be 1 micrometer in diameter. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. 5. Planococcus sp. The general features of Planococcus sp. Both sources have specific classification percentages that confer certainty. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. Sarcina, Catalase positive:Deinococcus,Marinococcus
Micrococcus varians The purity of the isolate was confirmed with the microscopic observation of a mono-culture. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. Micrococcus,
The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. Melissococcus
Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. The SPAdes Genome Assembler provided the overall result of the genomes assembly. Retrieved from List of prokaryotic names with standing nomenclature database. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. The species classification with the highest query cover is poorly documented in literature. Image 5 and 6 are from the Kraken app. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. Gram Positive Bacteria. Your email address will not be published. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. Based on multiple This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. However these similarities are not enough to convince me that they are the same species. Gram‐positive to Gram‐variable. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). 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